| 质粒名称: | pB-actin E6 E7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13712 |
| 存储实验室: | Karl Munger |
|
载体骨架基本信息 |
|
| 载体名称: | pB-actin |
| 载体出品公司: | |
| 空载体大小: | 7500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HPV 16 E6 E7,E6, E7 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 730 bp |
| 基因突变: | -- |
| GenBank编号 | NC_001526 |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Sal 1 (未破坏) |
| 3’克隆位点: | HindIII (未破坏) |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pB-actin E6 E7 was a gift from Karl Munger (Addgene plasmid # 13712 ; http://n2t.net/addgene:13712 ; RRID:Addgene_13712) |
| 参考文献部分: | The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R.. J Virol. 1989 Oct . 63(10):4417-21. |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pB-actin E6 E7 8876 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pB-actin E6 E7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8876)
AUTHORS Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R.
TITLE The E6 and E7 genes of the human papillomavirus type 16 together are
necessary and sufficient for transformation of primary human
keratinocytes.
JOURNAL J Virol. 1989 Oct . 63(10):4417-21.
REFERENCE 2 (bases 1 to 8876)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8876
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(3897..3914)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4068..4656)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4148..4167)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4827..5687)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5450..5469
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5688..5792)
/gene="bla"
/label=AmpR promoter
primer_bind 5860..5878
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc ccaccccacc
61 tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg gtggggggcc
121 cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg gcactgccca
181 gcgtggggcg cgggggccac ggcgcgcgcc cccagccccc gggcccagca ccccaaggcg
241 gccaacgcca aaactctccc tcctcctctt cctcaatctc gctctcgctc tttttttttt
301 tcgcaaaagg aggggagagg gggtaaaaaa atgctgcact gtgcggcgaa gccggtgagt
361 gagcggcgcg gggccaatca gcgtgcgccg ttccgaaagt tgccttttat ggctcgagcg
421 gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc caccaccgcc gagaccgcgt
481 ccgccccgcg agcacagagc ctcgcctttg ccgatccgcc gcccgtccac acccgccgcc
541 aggtaagccc ggccagccga ccggggcatg cggccgcggc cccttcgccc gtgcagagcc
601 gccgtctggg ccgcagcggg gggcgcatgg ggggggaacc ggaccgccgt ggggggcgcg
661 ggagaagccc ctgggcctcc ggagatgggg gacaccccac gccagttcgg aggcgcgagg
721 ccgcgctcgg gaggcgcgct ccgggggtgc cgctctcggg gcgggggcaa ccggcggggt
781 ctttgtctga gccgggctct tgccaatggg gatcgcaggg tgggcgcggc gtagcccccg
841 ccaggcccgg tgggggctgg ggcgccattg cgcgtgcgcg ctggtccttt gggcgctaac
901 tgcgtgcgcg ctgggaattg gcgctaattg cgcgtgcgcg ctgggactca aggcgctaat
961 tgcgcgtgcg ttctggggcc cggggtgccg cggcctgggc tggggcgaag gcgggctcgg
1021 ccggaagggg tggggtcgcc gcggctcccg ggcgcttgcg cgcacttcct gcccgagccg
1081 ctggccgccc gagggtgtgg ccgctgcgtg cgcgcgcgcc gacccggcgc tgtttgaacc
1141 gggcggaggc ggggctggcg cccggttggg agggggttgg ggcctggctt cctgccgcgc
1201 gccgcgggga cgcctccgac cagtgtttgc cttttatggt aataacgcgg ccggcccggc
1261 ttcctttgtc cccaatctgg gcgcgcgccg gcgccccctg gcggcctaag gactcggcgc
1321 gccggaagtg gccagggcgg gggcgacttc ggctcacagc gcgcccggct attctcgcag
1381 gatcagtcga ctctagagga tccttttatg caccaaaaga gaactgcaat gtttcaggac
1441 ccacaggagc gacccagaaa gttaccacag ttatgcacag agctgcaaac aactatacat
1501 gatataatat tagaatgtgt gtactgcaag caacagttac tgcgacgtga ggtatatgac
1561 tttgcttttc gggatttatg catagtatat agagatggga atccatatgc tgtatgtgat
1621 aaatgtttaa agttttattc taaaattagt gagtatagac attattgtta tagtttgtat
1681 ggaacaacat tagaacagca atacaacaaa ccgttgtgtg atttgttaat taggtgtatt
1741 aactgtcaaa agccactgtg tcctgaagaa aagcaaagac atctggacaa aaagcaaaga
1801 ttccataata taaggggtcg gtggaccggt cgatgtatgt cttgttgcag atcatcaaga
1861 acacgtagag aaacccagct gtaatcatgc atggagatac acctacattg catgaatata
1921 tgttagattt gcaaccagag acaactgatc tctactgtta tgagcaatta aatgacagct
1981 cagaggagga ggatgaaata gatggtccag ctggacaagc agaaccggac agagcccatt
2041 acaatattgt aaccttttgt tgcaagtgtg actctacgct tcggttgtgc gtacaaagca
2101 cacacgtaga cattcgtact ttggaagacc tgttaatggg cacactagga attgtgtgcc
2161 ccatctgttc tcagaaacca taatctacca tggctgatcc tgcaggtacc ggatccggta
2221 ccgagctcga attcaagctt ggaattctca tgtttgacag cttatcatcg ataagctact
2281 caggatttaa aaactggaac ggtgaaggtg acagcagtcg gttggagcga gcatccccca
2341 aagttcacaa tgtggccgag gactttgatt gcacattgtt gtttttttaa tagtcattcc
2401 aaatatgaga tgcattgtta caggaagtcc cttgccatcc taaaagccac cccacttctc
2461 tctaaggaga atggcccagt cctctcccaa gtccacacag gggaggtgat agcattgctt
2521 tcgtgtaaat tatgtaatgc aaaatttttt taatcttcgc cttaatactt ttttattttg
2581 ttttattttg aatgatgagc cttcgtgccc ccccttcccc cttttttgtc ccccaacttg
2641 agatgtatga aggcttttgg tctccctggg agtgggtgga ggcagccagg gcttacctgt
2701 acactgactt gagaccagtt gaataaaagt gcacacctta aaaatgaggc caagtgtgac
2761 tttgtggtgt ggctgggttg ggggcagcag agggtgaacc ctgcaggagg gtgaaccctg
2821 caaaagggtg gggcagtggg ggccaacttg tcctcaccca gagtgcaggt gtgtggagat
2881 ccctcctgcc ttgacattga gcagccttag agggtggggg aggctcaggg gtcaggtctc
2941 tgttcctgct tattggggag ttcctggcct ggcccttcta tgtctcccca ggtaccccag
3001 tttttctggg ttcacccaga gtgcagatgc ttgaggaggt gggaagggac tatttggggg
3061 tgtctggctc aggtgccatg cctcactggg gctggttggc acctgcattt cctgggagtg
3121 gggctgtctc agggtagctg ggcacggtgt tcccttgagt gggggtgtag tgagtgttcc
3181 tagctgccac gcctttgcct tcacctatgg gatcgtggct gtcagccttg agggtcagcc
3241 tggcccaggc tcccataggc ttaggagagg ccgcaattcc tacctgttca tccagacaga
3301 gggggacctg gaatcaaagt caagttgggg tggggggtcc atggggccat atctggcctg
3361 cagacagctc tggttagcta tgggctgagg tctggattct gccttgtgac tggagactgg
3421 gcgccatccc gtggcctctg agggcccagt ttgttcgtga cccatgctgc agagggagat
3481 acccaggctc ctggacctca gggcctgcct ggcactgcag gccaggttct ctttttattt
3541 ctgagacggg gtcttgtgtt gcccaggctg gagtgtaatg gcacaaccat ggctcactgc
3601 agcctcaact ttgggctcaa gcaattctcc tgcctcagcc tcccaagtag ctgaaactac
3661 acaggcatgc cccagcacgc ccagctagtt gcaggtcaga ttctcattgt tctacagggc
3721 cctgagttaa atactctcgc aagaagaaac caagcctggc ctgccttccc tctgctagaa
3781 gctgtgattg acaccagcag agaatgaagg aagaaaaggg ggtgaggatc tatgcggtgt
3841 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
3901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
3961 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
4021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
4081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
4141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
4201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
4261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
4321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
4381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
4441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
4501 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
4561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
4621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
4681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
4741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
4801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
4861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
4921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
4981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
5041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
5101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
5161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
5221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
5281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
5341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
5401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
5461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
5521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
5581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
5641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
5701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
5761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
5821 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
5881 tttcttatca tcgaattccg actttcaaac agcctttgga ctcaggcctt ggggacattc
5941 caggggaccc tttaccaggg aggggcagtg tgaggcagga ggcagggctg ccgcctcagg
6001 gaccctgagc ctagagtcct tcccaccgca tttggggact tgagcacttc accagtcctg
6061 actcagtttc ctagtctgtg aatgggggtg tgtctcctgg aatgttgccc cccctcattc
6121 attcattctc ctctcactga gcacctaccg tgttcagggt ccctgtcctc ctagagctaa
6181 ctcaagtctt ggagggaagc tctgtctcag ttccttcctc tacaaaatgg agcttgagga
6241 cgggcacggt ggctcatgcc tgtaatccca ggactttggg aggctgaggt gggaggatcg
6301 cttgagcccc ccggagctgg agaccagcct gggcaacatg gtgagaagcc atacctctac
6361 agataattta aaaattagcc aggcttggaa gtgtgtgcct atagtcccag ctactcgtga
6421 aggctttgag cccaggaggt cgaggctgca gtgagctgtg atcgcactcc agcctgggca
6481 tcacagcaag accttgtatc aaaaatagta ataataataa aaaggaggtt ggattccctc
6541 ctggcaggat agggagggcg ctgcagtgcc cagggcaagg tggctgggtg gttgttttgc
6601 gggagggcca aggagtggtc cctgggtctg cgctgtaaga gttggttgcc tggaggcctt
6661 gcagggtggg ggtgtcacca aagcaaaggc ttggagggga aaaaacaaaa gtcccagcca
6721 ggcaggtgga gtccccttgg ctgagttcaa ccgagggttc tccgggggct gcgtgcgtgc
6781 cccagtgaca gctccgaaag ctcccttaca gggcaaagtt cccaagcaca gaagagaacc
6841 tgttcacttc tcccctgctc ggcccgcccc ctggccaggc acctctactt cctcttttcc
6901 tgctccgctg cttgctttct ctcttcagct cctccctgcc cctcacccca ggctgctcgg
6961 ccacctccaa cctgccacct gaggacaccc aggcagtcac tcattcaaca gcgaggagcc
7021 ctggggtggg tgtagtggga aggagtgggg gtgacggaga ccctgggagg gctcgcagcc
7081 tggtggctga ggcccagttc taaatgccag ctgcaagcct tggtctgagg tagggaggaa
7141 ggcgtggctg cagaggctaa aacgcttccc caaagagggg ctttctggga tgggacttga
7201 agggtgcata ggagagcact aggaagtggc cgctgcagac agagggaacc acaagccagg
7261 aggacaggcc aggaatgctg cagcccgggg cggggtgggg ctggagctcc tgtctcttgg
7321 ccagctgaat ggaggcccag cggcaacaca ggtcctgcct ggggatcagg tctgctctgc
7381 accccacctt gctgcctgga gccgcccacc tgacaacctc tcatccctgc tctgtagatc
7441 cggtcccatc cccactgccc accccacccc cccagcactc cacccagttc aacgttccac
7501 gaacccccag aaccagccct catcaacagg cagcaagaag ggccccccgc ccatcgcccc
7561 acaacgccag ccgggtgaac tgtagcgttg gcaggtcctg aggcagctgg caagacgcct
7621 gcagctgaaa gatacaaggc cagggacagg acagtcccat ccccaggagg cagggagtat
7681 acaggctggg gaagtttgcc cttgcgtggg gtggtgatgg aggaggctca gcaagtcttc
7741 tggactgtga acctgtgtct gccactgtgt gctgggtggt ggtcatcttt cccaccaggc
7801 tgtggcctct gcaaccttca agggaggagc aggtcccatt ggctgagcac agccttgtac
7861 cgtgaactgg aacaagcagc ctccttcctg gccacaggtt ccatgtcctt atatggactc
7921 atctttgcct attgcgacac acactcaatg aacacctact acgcgctgca aagagccccg
7981 caggcctgag gtgcccccac ctcaccactc ttcctatttt tgtgtaaaaa tccagcttct
8041 tgtcaccacc tccaaggagg gggaggagga ggaaggcagg ttcctctagg ctgagccgaa
8101 tgcccctctg tggtcccacg ccactgatcg ctgcatgccc accacctggg tacacacagt
8161 ctgtgattcc cggagcagaa cggaccctgc ccacccggtc ttgtgtgcta ctcagtggac
8221 agacccaagg caagaaaggg tgacaaggac agggtcttcc caggctggct ttgagttcct
8281 agcaccgccc cgcccccaat cctctgtggc acatggagtc ttggtcccca gagtccccca
8341 gcggcctcca gatggtctgg gagggcagtt cagctgtggc tgcgcatagc agacatacaa
8401 cggacggtgg gcccagaccc aggctgtgta gacccagccc ccccgccccg cagtgcctag
8461 gtcacccact aacgccccag gcctggtctt ggctgggcgt gactgttacc ctcaaaagca
8521 ggcagctcca gggtaaaagg tgccctgccc tgtagagccc accttccttc ccagggctgc
8581 ggctgggtag gtttgtagcc ttcatcacgg gccacctcca gccactggac cgctggcccc
8641 tgccctgtcc tggggagtgt ggtcctgcga cttctaagtg gccgcaagcc acctgactcc
8701 cccaacacca cactctacct ctcaagccca ggtctctccc tagtgaccca cccagcacat
8761 ttagctagct gagccccaca gccagaggtc ctcaggccct gctttcaggg cagttgctct
8821 gaagtcggca agggggagtg actgcctggc cactccatgc cctccaagag ctcctt
//
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